SrasV1, Main, Exploration, bibRecord, 004D99

Intranasal immunization with inactivated SARS-CoV (SARS-associated coronavirus) induced local and serum antibodies in mice

Identifieur interne : 004D99 ( Main/Exploration ); précédent : 004D98; suivant : 004E00

Intranasal immunization with inactivated SARS-CoV (SARS-associated coronavirus) induced local and serum antibodies in mice

Auteurs : DI QU [République populaire de Chine] ; BOJIAN ZHENG [Hong Kong] ; XIN YAO [République populaire de Chine] ; YI GUAN [Hong Kong] ; Zheng-Hong Yuan [République populaire de Chine] ; Nan-Shan Zhong [République populaire de Chine] ; Li-Wei Lu [Hong Kong] ; Jian-Ping Xie [République populaire de Chine] ; Yu-Mei Wen [République populaire de Chine]

Source :

Vaccine [ 0264-410X ] ; 2005.

RBID : Pascal:05-0075987

Descripteurs français

Pascal (Inist)
- Virus syndrome respiratoire aigu sévère, Souris, Voie intranasale, Immunisation, Vaccin, Sérum, Anticorps, Syndrome respiratoire aigu sévère.
Wicri :
- topic : Vaccin.

English descriptors

KwdEn :
- Antibody, Immunization, Intranasal administration, Mouse, Serum, Severe acute respiratory syndrome, Severe acute respiratory syndrome virus, Vaccine.

Abstract

SARS-CoV (severe acute respiratory syndrome-associated coronavirus) strain GZ50 was partially purified and inactivated with 1:2000 formaldehyde. In cell culture the inactivated virus blocked the replication of live virus by decreasing the TCID_5.0 of the live virus 10^3.6 to 10^4.6 times. Inactivated GZ50 was used to immunize mice intranasally either alone, or after precipitation with polyethylene glycol (PEG), or with CpG, or CTB as an adjuvant. The titer of serum neutralizing antibodies was up to 1:640. In mice immunized with adjuvants or PEG precipitated GZ50, specific IgA was detected in tracheal-lung wash fluid by immunofluorescence. Though serum antibodies were detected, no anti-SARS-IgA could be detected in mice immunized only with inactivated GZ50. The roles of adjuvants in intranasal immunization with inactivated. SARS-CoV is discussed.

Affiliations:

Hong Kong, République populaire de Chine

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Le document en format XML

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<profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Antibody</term>
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<front><div type="abstract" xml:lang="en">SARS-CoV (severe acute respiratory syndrome-associated coronavirus) strain GZ50 was partially purified and inactivated with 1:2000 formaldehyde. In cell culture the inactivated virus blocked the replication of live virus by decreasing the TCID<sub>5.0</sub>
 of the live virus 10<sup>3.6</sup>
 to 10<sup>4.6</sup>
 times. Inactivated GZ50 was used to immunize mice intranasally either alone, or after precipitation with polyethylene glycol (PEG), or with CpG, or CTB as an adjuvant. The titer of serum neutralizing antibodies was up to 1:640. In mice immunized with adjuvants or PEG precipitated GZ50, specific IgA was detected in tracheal-lung wash fluid by immunofluorescence. Though serum antibodies were detected, no anti-SARS-IgA could be detected in mice immunized only with inactivated GZ50. The roles of adjuvants in intranasal immunization with inactivated. SARS-CoV is discussed.</div>
</front>
</TEI>
<affiliations><list><country><li>Hong Kong</li>
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<name sortKey="Zhong, Nan Shan" sort="Zhong, Nan Shan" uniqKey="Zhong N" first="Nan-Shan" last="Zhong">Nan-Shan Zhong</name>
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<country name="Hong Kong"><noRegion><name sortKey="Bojian Zheng" sort="Bojian Zheng" uniqKey="Bojian Zheng" last="Bojian Zheng">BOJIAN ZHENG</name>
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<name sortKey="Yi Guan" sort="Yi Guan" uniqKey="Yi Guan" last="Yi Guan">YI GUAN</name>
</country>
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</record>

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Intranasal immunization with inactivated SARS-CoV (SARS-associated coronavirus) induced local and serum antibodies in mice

Intranasal immunization with inactivated SARS-CoV (SARS-associated coronavirus) induced local and serum antibodies in mice

Source :

Descripteurs français

English descriptors

Abstract

Links toward previous steps (curation, corpus...)

Le document en format XML

Pour manipuler ce document sous Unix (Dilib)

Pour mettre un lien sur cette page dans le réseau Wicri